UV-visible-NIR spectroscopy of microscopic samples

Textile Fiber in a Microspectrophotometer

Microspectrophotometer

The UV-visible-NIR microspectrophotometer is a scientific instrument used to measure the spectra of microscopic samples.  For example, a forensic scientist will use one to analyze the dye in a single textile fiber, shown on the left, while a chemist will use it to measure the spectrum of a single microscopic crystal.  The microspectrophotometer combines a special optical microscope with a UV-visible-NIR range spectrophotometer.  The standard spectrophotometer is designed to measure samples on the order of 1 x 1 centimeters.  However, the microspectrophotometer is able to measure samples on the order of 1 x 1 micrometers...much smaller than the thickness of a human hair.

As shown in the diagram on the left, the instrument combines a special optical microscope with an optical spectrophotometer.  The instrument in this figure is configured for transmission microspectroscopy.  In this case, the light from the lamp housing is focused onto the sample on the microscope stage (I0).  The light that is transmitted through the sample, is collected by the objective (I) and focused onto the spectrophotometer entrance aperture. 

The UV-visible-NIR microspectrophotometer is a very flexible instrument and can be configured to measure the transmittance, absorbance, reflectance, polarization, fluorescence and photoluminescence microspectra™ of sample areas smaller than a micron. Microspectrophotometers are also capable of non-contact microspot thin film thickness measurement and colorimetry. Because UV-visible-NIR microspectrometers are so flexible, they are used in many fields of research and industry.

There are also different types of microspectrophotometers.  Some of these instruments are designed to be added to standard microscopes or probe stations, such as the 508 PV™ microscope spectrophotometer, while others are fully integrated, purpose built instruments such as the 20/30 PV™ microspectrophotometer. As such, microspectrophotometers have greater spectral ranges, better results and a number of features that are not possible with add-on units.

For more detail on microspectrophotometers, visit the following pages:

The Science of Microspectrophotometry

Microspectrophotometer Design

Microspectrophotometer Operation

How are Microspectrophotometers Used?

Units of UV-Visible-NIR Microspectroscopy

Algorithms of UV-Visible-NIR Microspectroscopy

Why use a Microspectrophotometer?

Why Use a Microspectrometer

The microspectrophotometer allows the scientist or engineer to acquire spectra of extremely small sample areas non-destructively. Measurements can be made while light is transmitted through the sample, reflected from it or even when the sample is made to emit light such as from an OLED pixel. The UV-visible-NIR range is especially important as more substances...even colorless ones...absorb in the UV than in the visible and infrared regions. Therefore, a UV microscope spectrometer is very useful for analysis of most samples for any application.

Microspectrophotometers are easily employed in many different fields and are found in both scientific laboratories and production facilities. In the production environment, for example, they are used for quality control of everything from color masks in flat panel displays to the thickness of films on semiconductor integrated circuits. Microspectrometers are used by analytical laboratories to identify and quantify microscopic samples ranging from the microfluidic kinetics, matching fibers or paints by a forensic chemist, the qualification of gems or coal by a geologist, the determination of the color of ink or paint by a process chemist and even the analysis of great works of art by conservators. As such, the microspectrometer is a highly flexible instrument with many different applications.