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Vitrinite Coal
The science
behind the microscope
photometer
Applications of the
microphotometer
Units of the microphotometer
CRAIC
QDI MP1™ Microscope
Photometer
General
diagram of a
Transmission Microphotometer
Immunofluorescence |
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The microscope photometer
is designed to measure the
intensity of electromagnetic
radiation from the UV,
through the visible to the
near infrared regions.
It can be configured for
single, double or triple
wavelength ratiometric
measurements of micron sized
samples. The measured
light can either have been
transmitted through the
sample, been reflected from
the sample or been emitted
from the sample by a
luminescent process.
Transmission or Absorbance
Micro Photometry
The
microscope photometer,
also called a cytophotometer, combines aspects of
both the microscope and a
photometer. When the
two components are
integrated, the system is
able to measure the
intensity of light and to
image microscopic samples.
Individually, the microscope
is an optical instrument
that uses lenses and mirrors
to produce magnified images
of microscopic objects or
microscopic areas of larger
objects. The sample
can be illuminated in a
number of ways.
Transmittance involves
focusing the light through a
condenser and onto the
sample.
The light that is
transmitted through the
sample is collected by the
microscope objective and
imaged onto entrance
aperture of the photometer.
The light intensity for the
selected wavelength is then
measured. The digital
camera images both the
aperture and the sample
simultaneously making it
simple to determine what
exactly is being measured.
Reflectance Micro Photometry
When the microscope is
configured for incident
illumination or reflectance,
the light is focused through
the objective and onto the
sample. The sample
reflects some light back and
this is collected by the
microscope objective.
That light is then imaged
onto entrance aperture of
the photometer and the
intensity for the selected
wavelength is then measured.
The digital camera images
both the aperture and the
sample simultaneously making
it simple to determine what
exactly is being measured.
Fluorescence Micro
Photometry
When the microscope is
configured for fluorescence
photometry, the exciting
energy is focused through
the objective and onto the
sample. The sample
absorbs the electromagnetic
energy and then re-emits a
lower energy photon.
some light back and this is
collected by the microscope
objective. That light
is then imaged onto entrance
aperture of the photometer
and the intensity for the
selected wavelength is then
measured. The digital
camera images both the
aperture and the sample
simultaneously making it
simple to determine what
exactly is being measured. |
