The Basics of UV-Visible-NIR Microspectrophotometry

UV-Visible-NIR microspectrophotometry is a non-destructive method used to measure the spectra of microscopic samples or microscopic areas. Depending on the setup, a microspectrophotometer may obtain a wide range of signal types from sample areas, including absorbance, fluorescence, polarization, reflectance and transmittance. 

This post covers the basics of UV-Visible-NIR microspectrophotometry, including how the microspectrophotometer works.

What is UV-Visible-NIR Microspectrophotometry?

UV-Visible-NIR microspectrophotometry is used to analyze and measure the optical properties of microscopic samples without damaging or even coming into contact with the sample’s surface. It is a crucial method that eclipses both conventional microscopy and photometry, with an array of practical uses. Common applications of UV-Visible-NIR microspectrophotometry include thin film thickness measurements and color space determination.

UV-Visible-NIR Microspectrophotometers

UV-Visible-NIR microspectrophotometers are flexible instruments that are used in a range of research and industry fields, including art, geology, materials science, pharmaceuticals and more. They are built by combining a microscope's excellent magnification capabilities and a spectrophotometer's spectral analysis to measure spectra on a microscopic scale. Samples can be viewed with high-resolution imaging from deep ultraviolet (UV) through to the near-infrared (NIR).
Microspectrophotometers can often be enhanced to be more effective by optimizing optics and light sources–processes that involve changing the source of light and the imaging systems. 

How Does A UV-Visible-NIR Microspectrophotometer Work?

A UV-Visible-NIR Microspectrophotometer is used to measure the spectra of microscopic samples. Sometimes, a microspectrophotometer will be added to a standard microscope, but others are fully integrated instruments.
When measuring the spectra, the microspectrophotometer will work in the following ways:

CRAIC Technologies

CRAIC Technologies™ manufactures a range of microspectrophotometers for a number of applications, as well as imaging microphotometers, UV-Visible-NIR microscopes, Raman microspectrometers and more. Below we will outline our most sophisticated product.

2030PV PRO™ microspectrophotometer

The most powerful micro-analysis instrument that CRAIC Technologies™ manufactures is the cutting-edge 2030PV PRO™ microspectrophotometer. It can be built to your requirements and uses the latest technology in electrics, optics, software and spectroscopy to provide outstanding performance and unique analyzing capabilities.

The 2030PV PRO™ is easy to use and the system includes a range of features such as automation, instruments ergonomics and software. In addition to the above, it is worth noting that CRAIC Technologies™ is the only certified company for NIST traceable microspectrometer standards.

If you are interested in learning more about our products or would like to make a purchase, please don’t hesitate to contact us today!