Algorithms used for Microspectroscopy

Light is transmitted through the sample, collected by the objective and focused onto the aperture and simulaneously an image and spectrum are collected.


Transmission spectrum from a microspectrophotometer.

Transmittance

Transmittance calculates a transmission spectrum from a sample and reference single-beam spectra.

From Two Single-Beam Spectra a transmission spectrum using the following formula:

Percent transmission calculation

where R is the single beam spectrum of the reference material, S is the single beam spectrum of the sample and D is the dark counts of the system.

From Absorbance Data a transmission spectrum can be generated using the following formula:

Convert absorbance to percent transmission.

where A is the absorbance value. This is a calculation from data that has already been collected.


Absorbance

Absorbance calculates an absorption spectrum from a sample and reference single-beam spectra or from a transmission spectrum.

From Two Single-Beam Spectra an absorption spectrum can be generated using the following formula:

Absorbance calculation

where R is the single beam spectrum of the reference material, S is the single beam spectrum of the sample and D is the dark counts of the system.

From Transmission Data an absorption spectrum can be generated using the following formula:

Convert percent transmission to absorance.

where %T is the percent transmittance value. This is a calculation from data that has already been collected.


Reflectance

Reflectance calculates a reflectance spectrum from a sample and reference single-beam spectra or from an log 1/R spectrum.

From Two Single-Beam Spectra a reflectance spectrum can be generated using the following formula:

Percent reflectance calculation.

where R is the single beam spectrum of the reference material, S is the single beam spectrum of the sample and D is the dark counts of the system.

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