The Basics of UV-Visible-NIR Microspectrophotometry

UV-Visible-NIR microspectrophotometry is a non-destructive method used to measure the spectra of microscopic samples or microscopic areas. Depending on the setup, a microspectrophotometer may obtain a wide range of signal types from sample areas, including absorbance, fluorescence, polarization, reflectance and transmittance. 

5 Challenges Resolved Using Microspectroscopy

Microspectroscopy, also known as microspectrophotometry, is a powerful imaging tool that combines the microscale imaging capabilities of a conventional microscope with the analytical capabilities of a spectrometer. Essentially, a microspectrophotometer is used to measure the molecular spectral emissions of samples on much smaller scales than a conventional spectrometer. This enables detailed materials characterization at the microscopic level. Naturally, microspectroscopy can help researchers resolve numerous challenges in research and production applications.

How Does a Microspectrophotometer Work?

Spectrophotometry is a powerful analytical tool used to measure the luminous intensity of various samples across a measurement area of around 1 x 1 centimetres (cm). But what if you want to observe the spectra of microscopic samples? Standard microscopes image samples using visible light with a maximum optical resolving power of two micrometres (μm). However, it is possible to add spectroscopy to UV-visible-NIR optical microscopy to observe spectra and images at high resolutions (1 x 1 μm).

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