MicroRaman spectroscopy is a light scattering technique that utilizes a Raman microscope spectrometer to analyze live cells. The analysis is conducted by identifying molecules and their functional groups. It is a technique based on Raman spectroscopy, where the interaction between light and matter is studied after the light has been scattered. MicroRaman spectroscopy utilizes a more specific Raman spectrometer, and throughout this blog post, we will look at the equipment associated with MicroRaman spectroscopy in-depth.
What is MicroRaman Spectroscopy?
In Raman spectroscopy, Raman scattered light provides data for the vibrational modes of the sample molecules, which is then used to identify molecules and their functional groups. This works using a spectrometer where a single wavelength of light is projected onto a sample. The photons from the light source interact with the sample molecules, then the photons are inelastically scattered.
MicroRaman spectroscopy, or Raman microspectroscopy, differs in that a Raman microscope spectrometer has an integrated optical microscope which allows the analyst to retrieve Raman spectra of microscopic samples. The benefits of using a Raman microscope spectrometer are that it is non-invasive, and the samples do not require any preparation. Other advantages include the following:
- A range of materials can be analyzed with Raman spectroscopy, including liquids, polymers, solids and vapors.
- Large quantities of samples are not required.
- Quick process and acquisition of spectra.
- Some effects can be enhanced in localized regions of the sample.
Which Applications Is MicroRaman Spectroscopy Used In?
MicroRaman spectroscopy can be used in many applications. From analyzing minute microbiological materials to identifying dyes and pigments in forensic examinations, it can be an extremely useful method.
In addition to those mentioned above, a Raman microscope spectrometer can also be used to carry out the following, plus many more:
- Assessing bioactivity of biomaterials
- Contaminant idenfication
- Drug discovery
- Dye identifcation (textiles)
- OLED development
- Tissue engineering
- Thin-film characterization
MicroRaman Spectroscopy with CRAIC Technologies
CRAIC Technologies designs, develops and manufactures a wide range of spectrometers for a number of applications. Please see the below for our flagship Raman microscope spectrometer and its key features.
Apollo M™ Confocal Raman microspectrometer
The Apollo M™ Raman microscope spectrometer is a high-performance system that is robust, reliable and powerful. It can be used alone or in conjunction with our UV-VIS-NIR Microspectrophotometer. The Apollo M™ is the perfect instrument for ease of use and fast acquisition of spectra, leaving the analysts to focus on sample measurements and not on learning to operate the machinery.
Other features include:
- Easy to maintain
- High sensitivity and speed
- Multiple wavelengths available (405,532, 633, 785 and 830 nm)
- Supports Raman spectral mapping
If you are looking to purchase a Raman microscope spectrometer, or require any further information on any of our products, please contact us via the contact form and a team member will be in touch shortly.