Spectrophotometry with A Microscope
Emission Spectra Measured with a Microscope Spectrophotometer
Microscope spectrophotometry is the measurement of UV-vsibile-NIR spectra of microscopic samples.
Microscope Spectrophotometry uses instruments designed to measure UV-visible-NIR spectra of microscopic samples or microscopic areas of larger objects. There are two basic types: the fully integrated microspectrophotometer (as shown) that has been built and optimized for microspectrophotometry. There is also the spectrophotometer unit designed to attach to an open photoport of an optical microscope. Each has its strengths and depending upon the configuration, both are capable of measuring the spectra of microscopic samples by transmission, absorbance, reflectance, fluorescence, emission and polarization spectrophotometry. With special software, both are capable of 3D surface mapping measurements and colorimetry as well.
A CRAIC Technologies™ microscope spectrophotometer is a purpose-built system that allows UV-visible-NIR range micro spectrophotometry both non-destructively and with no sample contact. Capable of analyzing even sub-micron areas, they are also capable of high resolution digital imaging. Designed for ease-of-use, they are durable instruments designed for microscale spectroscopy.
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