Spectrophotometry with A Microscope

Microscope Spectrometer

 

 

Micro spectrophotometry data

Emission Spectra Measured with a Microscope Spectrophotometer

 

 

 

Microscope spectrophotometry is the measurement of UV-vsibile-NIR spectra of microscopic samples.

Microscope Spectrophotometry uses instruments designed to measure UV-visible-NIR spectra of microscopic samples or microscopic areas of larger objects.  There are two basic types: the fully integrated microspectrophotometer (as shown) that has been built and optimized for microspectrophotometry.  There is also the spectrophotometer unit designed to attach to an open photoport of an optical microscope.   Each has its strengths and depending upon the configuration, both are capable of measuring the spectra of microscopic samples by transmission, absorbance, reflectance, fluorescence, emission and polarization spectrophotometry.   With special software, both are capable of 3D surface mapping measurements and colorimetry as well.

A CRAIC Technologies™ microscope spectrophotometer is a purpose-built system that allows UV-visible-NIR range micro spectrophotometry both non-destructively and with no sample contact.  Capable of analyzing even sub-micron areas, they are also capable of high resolution digital imaging.  Designed for ease-of-use, they are durable instruments designed for microscale spectroscopy.

 

 

Contact CRAIC Today!

 

 

 

To learn more about microscope spectrophotometers, select a link:

What is a Microscope Spectrophotometer?

Science of Micro Spectrophotometry

Microscope Spectrophotometer Design

Uses of Microscope Spectrophotometers

508 PV™ Microscope Spectrophotometers

20/30 PV™ Microspectrophotometer

CRAIC Apollo™ Raman Microspectrometer

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

We invite you to discover our revolutionary technologies that include a range of microscope spectrometers, microspectrometers, UV-visible-NIR microscopes, Raman microspectrometers, Traceable Standards, micro spectrometry accessories and software. We further invite you to experience our exceptional service and technical support.

 

 

Spectroscopy with a Microscope

Microscope Spectrometer

 

 

Micro spectroscopy data

Emission Spectra Measured with a Microscope Spectrophotometer

 

 

Microscope spectroscopy is the measurement of UV-vsibile-NIR spectra of microscopic samples.

Microscope spectroscopy uses instruments designed to measure UV-visible-NIR spectra of microscopic samples or microscopic areas of larger objects.  There are two basic types: the fully integrated microspectrophotometer (as shown) that has been built and optimized for microspectrophotometry.  There is also the spectrophotometer unit designed to attach to an open photoport of an optical microscope.   Each has its strengths and depending upon the configuration, both are capable of measuring the spectra of microscopic samples by transmission, absorbance, reflectance, fluorescence, emission and polarization spectroscopy.   With special software, both are capable of thin film thickness measurements and colorimetry as well.

A CRAIC Technologies™ microscope spectrophotometer is a purpose-built system that allows UV-visible-NIR range micro spectroscopy both non-destructively and with no sample contact.  Capable of analyzing even sub-micron areas, they are also capable of high resolution digital imaging.  Designed for ease-of-use, they are durable instruments designed for microscale spectroscopy.

Contact CRAIC Today!

To learn more about microscope spectrophotometers, select a link:

What is a Microscope Spectrophotometer?

Science of Microspectroscopy

Microscope Spectrophotometer Design

Uses of Microscope Spectrophotometers

508 PV™ Microscope Spectrophotometers

20/30 PV™ Microspectrophotometer

CRAIC Apollo™ Raman Microspectrometer

 

We invite you to discover our revolutionary technologies that include a range of microscope spectrometers, microspectrometers, UV-visible-NIR microscopes, Raman microspectrometers, Traceable Standards, micro spectrometry accessories and software. We further invite you to experience our exceptional service and technical support.

 

 

Learn about the uses of Spectrometry with a Microscope

Microscope Spectrometer

 

 

Micro spectrometry data

Emission Spectra Measured with a Microscope Spectrometer

 

 

 

Microscope Spectrometry is the measurement of UV-vsibile-NIR spectra of microscopic samples.

Microscope Spectrometry uses instruments designed to measure UV-visible-NIR spectra of microscopic samples or microscopic areas of larger objects.  There are two basic types: the fully integrated microspectrometer (as shown) that has been built and optimized for microspectrometry.  There is also the spectrometer unit designed to attach to an open photoport of an optical microscope.   Each has its strengths and depending upon the configuration, both are capable of measuring the spectra of microscopic samples by transmission, absorbance, reflectance, fluorescence, emission and polarization spectrometry.   With special software, both are capable of 3D surface mapping measurements and colorimetry as well.

A CRAIC Technologies™ microscope spectrometer is a purpose-built system that allows UV-visible-NIR range micro spectrometry both non-destructively and with no sample contact.  Capable of analyzing even sub-micron areas, they are also capable of high resolution digital imaging.  Designed for ease-of-use, they are durable instruments designed for microscale spectroscopy.

 

 

Contact CRAIC Today!

 

 

To learn more about microscope spectrometers, select a link:

What is a Microscope Spectrometer?

Science of Micro Spectrometry

Microscope Spectrometer Design

Uses of Microscope Spectrometers

508 PV™ Microscope Spectrometers

20/30 PV™ Microspectrometer

CRAIC Apollo™ Raman Microspectrometer

  

 

We invite you to discover our revolutionary technologies that include a range of microscope spectrometers, microspectrometers, UV-visible-NIR microscopes, Raman microspectrometers, Traceable Standards, micro spectrometry accessories and software. We further invite you to experience our exceptional service and technical support.

 

 

Relative Intensity Measurements of Displays

Microdisplay

Figure 1: Microspectrometers are used to measure the intensity of each pixel


 Click to Learn More!

 

Microdisplays

Figure 2: Typical test spectra from a display. Intensities can be directly compared between pixels.

 

 

The luminance, intensities or brightness of individual pixels and groups of pixels can be measured and compared using microspectrometers.

Flat panel displays consist of a series of colored lighted areas arranged in a pattern and can only be readily viewed with magnifying optics.  Due to the high resolution of flat panel displays, they are manufactured with hundreds of rows of microscopic "pixels" on a surface.  Generally, these pixels are red, blue and green though pixels with other colors are also made...for example the latest four color systems also incorporate yellow pixels.

One aspect of display quality control consists of making sure that the brightness or intensity of each pixel does not vary across the entire display.  For example, all the green pixels should have the same brightness no matter where they are positioned in the display. 

Microspectrometers are able to gather relative intensity data from single pixels and from groups of pixels (if you are interested in mura).  Additionally, microspectrometers are also able to map the relative intensity variation within even a single pixel!  This leads to a new level of precision for improved displays.

The way a microspectrometer works is that spectrophotometer entrance aperture is placed over the pixel or pixels in questions.  The black square in Figure 1 is an example.  The spectra is then acquired and will look something like the three spectra in Figure 2.  Using the LambdaFire™ software, the intensity data is compared with measurements of other pixels to show that the brightness of each sampled area is within the manufacturing parameters.  A high resolution map of the relative intensities can then be generated.

Learn more about relative intensity measurements of displays below: 

What is a Microspectrometer?

Science of Microspectrometers


 

Microdisplay Inspection

Microdisplay

 


 

Microdisplays

Typical test spectra from a microdisplay

 

 

Microdisplay Inspection: Color, relative intensity, spectroscopy and film thickness of pixels and light sources.

Microdisplays consist of a series of colored lighted areas arranged in a pattern and can only be readily viewed with magnifying optics.  They are used as high resolution displays for everything from mobile phones to video display headgear to MP3 players.  There are many different designs but their small scale causes many quality control challenges. 

Due to the nature of microdisplays, they are manufactured with hundreds of rows of microscopic "pixels" on a surface.  These pixels can be smaller than 10 microns across.  Quality control of both the components and completed microdisplays is done by optical microspectroscopy.  Microspectrometers, such as those made by CRAIC Technologies, are used to measure the color and the intensity of the output from individual pixels and from groups of pixels.  In fact, CRAIC microspectrometers can be used to map the color and intensity outputs of individual pixels in addition to the entire microdisplay.  This is important as manufacturers of microdisplays need to make sure that all the different types of pixels are the same color and brightness.  A microspectrophotometer does this quickly and easily. 

To learn more about microdisplay testing:

What is a Microspectrophotometer?

Science of Microspectrophotometers

Colorimetry of Pixels and Displays

Relative Intensity Measurements

508 PV™ Spectrophotometers for Microscopes and Probe Stations