Spectral microscopy!

Optical microscopes are routinely found in a wide variety of laboratories, but they are often only used as a magnifying glass to allow the user to better see the sample. However, even an inexpensive microscope can be upgraded to do so much more! By sending the light from the microscope to a spectrometer, many different types of spectra can be measured with a much smaller sampling area than is capable with a standard bench-top spectroscopy tool, such as transmission, reflection, fluorescence, and other types of optical emission. Spectral microscopy is done with microspectrophotometers which are designed to measure UV-VIS-NIR spectra of microscopic samples or microscopic areas of larger objects. While a standard spectrophotometer is designed to measure samples on the order of 1 x 1 centimeters, the microspectrophotometer is able to measure samples on the order of 1 x 1 micrometers...much smaller than the thickness of a human hair. The 508 PVTM microscope spectrophotometer is a modular head unit that can be installed on the open phototube port of most upright microscopes to add spectroscopy to your microscope. With CRAIC Technologies products, you can acquire high quality spectra of even sub-micron samples rapidly, non-destructively and with ease.

As shown in the diagram on the right, the MSP head unit can be added to the photoport of a microscope, and the spectrometer measures the light that is collected by the objective and focused onto the spectrophotometer entrance aperture.  Whether this light is transmitted through the sample, reflected from the sample, or emitted by the sample, that only changes the calculations done on the light intensity measured.

Beyond just measuring UV-VIS-NIR spectra with microscale sampling areas, spectral microscopy is also capable of microspot thin film thickness and colorimetry measurements. When combined with motorized stages, hyperspectral data cubes can even be created with various types of spectra.  Because these instruments are so flexible, MSP's are used in many diverse fields of research and industry.

Spectral microscope

Optical microscopes are routinely found in a wide variety of laboratories, but they are often only used as a magnifying glass to allow the user to better see the sample. However, even an inexpensive microscope can be upgraded to do so much more! By sending the light from the microscope to a spectrometer, many different types of spectra can be measured with a much smaller sampling area than is capable with a standard bench-top spectroscopy tool, such as transmission, reflection, fluorescence, and other types of optical emission. The spectral microscope (or microspectrophotometer / MSP) is designed to measure UV-VIS-NIR spectra of microscopic samples or microscopic areas of larger objects. While a standard spectrophotometer is designed to measure samples on the order of 1 x 1 centimeters, the microspectrophotometer is able to measure samples on the order of 1 x 1 micrometers...much smaller than the thickness of a human hair. The 508 PVTM microscope spectrophotometer is a modular head unit that can be installed on the open phototube port of most upright microscopes to add spectroscopy to your microscope. With CRAIC Technologies products, you can acquire high quality spectra of even sub-micron samples rapidly, non-destructively and with ease.

As shown in the diagram on the right, the MSP head unit can be added to the photoport of a microscope, and the spectrometer measures the light that is collected by the objective and focused onto the spectrophotometer entrance aperture.  Whether this light is transmitted through the sample, reflected from the sample, or emitted by the sample, that only changes the calculations done on the light intensity measured.

Beyond just measuring UV-VIS-NIR spectra with microscale sampling areas, microscope spectrophotometers are also capable of microspot thin film thickness and colorimetry measurements. When combined with motorized stages, hyperspectral data cubes can even be created with various types of spectra.  Because these instruments are so flexible, MSP's are used in many diverse fields of research and industry.

Colloidal Materials Research

Colloid development can be done with a microspectrophotometer

 

 

Colloidal Science can be done with microspectrophotometers

Microscopic Materials Science Spectroscopy

 

 

Microspectrophotometers are used to analyze colloids and colloidal materials by imaging and spectroscopy

A colloid is a substance that is evenly dispersed on the microscopic scale throughout another substance.  Colloidal substances have many different applications ranging from pharmaceuticals to Raman spectral enhancement materials.  Colloids can either be naturally derived or created by such processes as high shear techniques.  Due to their unique nature, they have many useful and interesting properties. 

Due to the microscopic scales of colloids and colloidal solutions, microspectrophotometers are the perfect tool.  Able to analyze micro-scale samples by absorbance, reflectance or even fluorescence, these tools are easy-to-use and very accurate.  Their flexibility and accuracy make them very importance for analyzing everything from the optical effects of butterfly wings to protein crystals. 

To learn more about microspectroscopy and colloid and colloidal research applications, select one of the following links: 

What is a Microspectrophotometer?

Science of Microspectrophotometers

Microspectrophotometer Design

Uses of Microspectrophotometers

20/30 PV™ Microspectrophotometers

Raman spectroscopy with a microscope!

Optical microscopes are routinely found in a wide variety of laboratories, but they are often only used as a magnifying glass to allow the user to better see the sample. But when CRAIC Technologies adds a laser and a Raman spectrometer, along with an advanced optical interface, you can do so much more! CRAIC Raman spectrometers are designed to measure Raman spectra of microscopic samples or microscopic areas of larger objects. While a standard Raman spectrometer is designed to measure samples on the order of 1 x 1 centimeters, the Raman microspectrometer is able to measure samples on the order of 1 x 1 micrometers...much smaller than the thickness of a human hair. The Apollo Raman systems are modular units that are installed on an upright optical microscopes to add Raman spectroscopy capabilities. With CRAIC Technologies products, you can acquire high quality Raman spectra of even sub-micron samples rapidly, non-destructively and with ease.

As shown in the diagram on the right, the Apollo Raman module is integrated with the frame of an upright optical microscope.  The module includes the lasers, the Raman spectrometer and integration optics.  The laser illuminates the sample and the Raman spectrometer measures the light that is collected by the objective and focused onto the Raman spectrometer entrance aperture. 

Of course, CRAIC Raman microspectrometers can also do much more.  They can also be configured to measure UV-VIS-NIR microspectra™ in transmission, reflectance, fluorescence and photoluminescence.  They are also capable of microspot thin film thickness and colorimetry measurements.

Raman spectroscopy with a microscope!

Optical microscopes are routinely found in a wide variety of laboratories, but they are often only used as a magnifying glass to allow the user to better see the sample. But when CRAIC Technologies adds a laser and a Raman spectrometer, along with an advanced optical interface, you can do so much more! CRAIC Raman spectrometers are designed to measure Raman spectra of microscopic samples or microscopic areas of larger objects. While a standard Raman spectrometer is designed to measure samples on the order of 1 x 1 centimeters, the Raman microspectrometer is able to measure samples on the order of 1 x 1 micrometers...much smaller than the thickness of a human hair. The Apollo Raman systems are modular units that are installed on an upright optical microscopes to add Raman spectroscopy capabilities. With CRAIC Technologies products, you can acquire high quality Raman spectra of even sub-micron samples rapidly, non-destructively and with ease.

As shown in the diagram on the right, the Apollo Raman module is integrated with the frame of an upright optical microscope.  The module includes the lasers, the Raman spectrometer and integration optics.  The laser illuminates the sample and the Raman spectrometer measures the light that is collected by the objective and focused onto the Raman spectrometer entrance aperture. 

Of course, CRAIC Raman microspectrometers can also do much more.  They can also be configured to measure UV-VIS-NIR microspectra™ in transmission, reflectance, fluorescence and photoluminescence.  They are also capable of microspot thin film thickness and colorimetry measurements.

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