Absorption Imaging from the UV to SWIR

Standard microscopes are used for increasing the capabilities of the human eye (i.e. magnifying samples for observation), and so they are designed with the visible spectrum in mind, using glass optics and substrates as well as light sources limited to visible wavelengths (~400-700 nm).  However, samples are not limited to only the visible wavelengths for absorption of light.  To better analyze samples outside of the visible wavelengths, CRAIC Technologies has developed a unique microscope featuring quartz optics and broadband light sources for high resolution imaging from the deep UV (200 nm) out to the NIR and SWIR (2,500 nm).  Microscopic protein crystals are just one example.  They are transparent in the visible range but can be easily seen at 280 nm due to the strong absorbance of certain amino acids.   Another advantage is in semiconductor metrology, where the longer wavelengths are not absorbed in the silicon substrates, but do interact with other compounds and layers.  This allows for non-destructive imaging inside of bonded silicon wafer and other semiconductor devices!

Another major advantage beyond the significantly larger spectral range is in terms of the increased spatial resolution possible by utilizing the shorter wavelengths.  By decreasing the imaging wavelength from the visible with an average of 550 nm to the deep UV at 250 nm, the resolving power is increased by over 2X! This often allows for visualizing smaller features that are ~200 nm in diameter without the use of immersion oils.

Learn more about UV-VIS-NIR-SWIR microscopes:

Contact CRAIC Today!

UV-visible-NIR microscopes, UV-visible-NIR microspectrometers and Raman microspectrometers are general purpose laboratory instruments. They have not been cleared or approved by the European IVD Directive, the United States Food and Drug Administration or any other agency for diagnostic, clinical or other medical use.

Ultraviolet Microscope

Ultraviolet Microscope

The Ultraviolet Microscope is a microscope that can image in the ultraviolet region. 

Ultraviolet microscopes are designed to "see" beyond what a standard optical microscope can image.  With upgraded ultraviolet optics, light sources and cameras, ultraviolet microscopes can image microscopic samples in the visible and the ultraviolet region.  This means that ultraviolet microscopes have features that make them superior to normal visible range microscopes:

  • Some materials are transparent or clear in normal microscopes but can be imaged with ultraviolet microscopes
  • By using the shorter wavelengths of ultraviolet light, higher image resolution can be obtained than with the longer wavelengths of visible range light.

One example is in imaging protein crystals: these microscopic crystals are transparent in the visible range but can be easily seen at 280 nm due to the strong absorbance of certain amino acids.  

CRAIC Technologies offers a number of solutions for the ultraviolet microscope.  These custom designed microscopes capable of imaging from the deep ultraviolet all the way into the near IR.  They are capable of ultraviolet microscopy in transmission, reflectance and even fluorescence.

Learn more about ultraviolet microscopes:

  Ultraviolet Microscope Design

Uses of the Ultraviolet Microscope

UVtM-1™ Ultraviolet Microscope

 
The lit microscope base is a trademark of CRAIC Technologies, Inc.
 UV-visible-NIR microscopes, UV-visible-NIR microspectrometers and Raman microspectrometers are general purpose laboratory instruments. They have not been cleared or approved by the European IVD Directive, the United States Food and Drug Administration or any other agency for diagnostic, clinical or other medical use.

 

 

UV Microscope

UV Microscope

The UV Microscope is a microscope that can image in the ultraviolet region. 

UV microscopes are designed to "see" beyond what a standard optical microscope can image.  With special UV optics, light sources and cameras, UV microscopes can image microscopic samples in the visible and the UV region.  This means that UV microscopes have features that make them superior to normal visible range microscopes:

  • Some materials are transparent or clear in normal microscopes but can be imaged with UV microscopes
  • By using the shorter wavelengths of UV light, higher image resolution can be obtained than with the longer wavelengths of visible range light.

One example is in imaging protein crystals: these microscopic crystals are transparent in the visible range but can be easily seen at 280 nm due to the strong absorbance of certain amino acids.  

CRAIC Technologies offers a number of solutions for the UV microscope.  These custom designed microscopes capable of imaging from the deep UV all the way into the near IR.  They are capable of UV microscopy in transmission, reflectance and even fluorescence.

Contact CRAIC Today!

Learn more about UV microscopes:

  UV Microscope Design

Uses of the UV Microscope

UVM-1™ UV Microscope

 
The lit microscope base is a trademark of CRAIC Technologies, Inc.
 UV-visible-NIR microscopes, UV-visible-NIR microspectrometers and Raman microspectrometers are general purpose laboratory instruments. They have not been cleared or approved by the European IVD Directive, the United States Food and Drug Administration or any other agency for diagnostic, clinical or other medical use.

 

Cytospectrometer

Cytospectrophotometer

 


 

Cytospectrophtometer

Emission Spectra Measured with a Microspectrophotometer

 

 

The Cytospectrometer measure the UV-vsibile-NIR spectra of sub-cellular components.

The Cytospectrometer is designed to measure UV-visible-NIR spectra of cellular components.  In other words, the cytospectrophotometer is a microspectrophotometer of which there are two basic types: the fully integrated microspectrophotometer that has been built and optimized for microspectroscopy and the spectrophotometer unit designed to attach to an open photoport of a biologicalal microscope.   Depending upon the configuration, both are capable of measuring the spectra of microscopic samples by transmission, absorbance, reflectance, fluorescence, emission and polarization microspectroscopy.   With special software, both are capable of surface spectral mapping measurements and colorimetry as well.

A CRAIC Technologies™ microspectrophotometer is a purpose-built system that allows you to analyze UV-visible-NIR range microspectra™ non-destructively and with no sample contact.  Capable of analyzing even sub-micron areas, they are also capable of high resolution digital imaging.  Designed for ease-of-use, they are durable instruments designed for microscale spectroscopy.

To learn more about microspectrophotometry and microspectrophotometers, select one of the following links: 

What is a Microspectrophotometer?

Science of Microspectrophotometry

Microspectrophotometer Design

Uses of Microspectrophotometers

20/30 PV™ Microspectrophotometer

508 PV™ Microscope Spectrophotometers


  

We invite you to discover our revolutionary technologies that include a range of microscope spectrophotometers, microspectrophotometers, UV-visible-NIR microscopes, Raman microspectrometers, Traceable Standards, micro spectrophotometry accessories and software. We further invite you to experience our exceptional service and technical support.

 

 

 

 UV-visible-NIR microscopes, UV-visible-NIR microspectrometers and Raman microspectrometers are general purpose laboratory instruments. They have not been cleared or approved by the European IVD Directive, the United States Food and Drug Administration or any other agency for diagnostic, clinical or other medical use.

Luminescence

Luminescence is the decay from the excited state to the ground state

Luminescence is the emission of a photon with the decay of an electron from an excited state to the ground state.  Different decay paths describe different types of luminescence.  For example, fluorescence occurs with the decay from the singlet S1 to the S0 ground state directly and is generally on the nanosecond time scale.  Phosphorescence occurs when the decay is from a triplet state to the S0 ground state and generally takes much longer. 

The excitation to the excited state occurs by absorption of energy.  The most common energy source is a light source.  However, chemical reactions (chemiluminescence), electricity (electroluminescence) and more can all act as means to achieve the excited energy state.

Luminescence spectrometers are designed to collect and measure the emitted photons.  The microspectrometer can be configured as a luminescence spectrometer which is combined with a microscope.  They are designed to measure  luminescent spectra of microscopic samples or microscopic areas of larger objects.  There are two basic types: the fully integrated microspectrophotometer that has been built and optimized for luminescent microspectroscopy and the spectrophotometer unit designed to attach to an open photoport of an optical microscope.   The beauty of microspectrometers is that they can also be configured to measure the transmission and reflectance spectra of microscopic sample areas in addition to luminescence.  And with special software, they are capable of colorimetry as well.

A CRAIC Technologies™ microspectrometer is a purpose-built system that allows you to analyze UV-visible-NIR range luminescent emissions non-destructively and with no sample contact.  Capable of analyzing even sub-micron areas, they are also capable of high resolution digital imaging.  Designed for ease-of-use, they are durable instruments designed for microscale spectroscopy.

To learn more about microspectroscopy and microspectrometers, select one of the following links: 

What is a Microspectrometer?

Microspectrometer Design

Uses of Microspectrometers

20/30 PV™ Microspectrophotometers