How Does MicroRaman Work?

MicroRaman spectroscopy is a light scattering technique that utilizes a Raman microscope spectrometer to analyze live cells. The analysis is conducted by identifying molecules and their functional groups. It is a technique based on Raman spectroscopy, where the interaction between light and matter is studied after the light has been scattered. MicroRaman spectroscopy utilizes a more specific Raman spectrometer, and throughout this blog post, we will look at the equipment associated with MicroRaman spectroscopy in-depth.

When to Use a Raman Microscope Spectrometer

Raman microscopy is an extremely powerful analytical method. Combining the unique chemical identification capabilities of Raman spectroscopy with traditional confocal microscopy opens an array of applications in a wide range of fields. But what is a Raman microscope spectrometer, and when might you use one?

The Basics of UV-Visible-NIR Microspectrophotometry

UV-Visible-NIR microspectrophotometry is a non-destructive method used to measure the spectra of microscopic samples or microscopic areas. Depending on the setup, a microspectrophotometer may obtain a wide range of signal types from sample areas, including absorbance, fluorescence, polarization, reflectance and transmittance. 

5 Challenges Resolved Using Microspectroscopy

Microspectroscopy, also known as microspectrophotometry, is a powerful imaging tool that combines the microscale imaging capabilities of a conventional microscope with the analytical capabilities of a spectrometer. Essentially, a microspectrophotometer is used to measure the molecular spectral emissions of samples on much smaller scales than a conventional spectrometer. This enables detailed materials characterization at the microscopic level. Naturally, microspectroscopy can help researchers resolve numerous challenges in research and production applications.

How Does a Microspectrophotometer Work?

Spectrophotometry is a powerful analytical tool used to measure the luminous intensity of various samples across a measurement area of around 1 x 1 centimetres (cm). But what if you want to observe the spectra of microscopic samples? Standard microscopes image samples using visible light with a maximum optical resolving power of two micrometres (μm). However, it is possible to add spectroscopy to UV-visible-NIR optical microscopy to observe spectra and images at high resolutions (1 x 1 μm).


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