How Does a Microspectrophotometer Work?

Spectrophotometry is a powerful analytical tool used to measure the luminous intensity of various samples across a measurement area of around 1 x 1 centimetres (cm). But what if you want to observe the spectra of microscopic samples? Standard microscopes image samples using visible light with a maximum optical resolving power of two micrometres (μm). However, it is possible to add spectroscopy to UV-visible-NIR optical microscopy to observe spectra and images at high resolutions (1 x 1 μm).

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